FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2289775
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9000.039.1997158
GCGTAAT1102.5350222E-432.734181
GTCTCGC8300.032.103161
GTCTTGC20650.031.7355141
CTGTCGC13000.031.5689989
TCGCTCT10500.030.1712074
GTATTAG8150.026.5086631
GTGTAAC7550.025.753781
GTATTCG1450.001275857424.8328291
GTATAGA7600.024.6367821
GTGTAAT10250.024.5905591
GGGTTTC39900.024.5465351
CGCTCTG11800.024.40665
GTACCGG2401.6957565E-624.0050661
GTATAAC4800.024.0050661
TTCACCG7500.023.9998255
TTGCTCT30350.023.2485944
CTCGCTC10850.023.2256373
TTACACT10550.023.2036234
GAGTGCA57200.023.0347986
Produced by FastQC (version 0.11.5)