FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3334350
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11600.053.9971738
GTCTCGC13600.047.175311
CTGTCGC14800.044.754419
TCGCTCT15200.044.0503234
TCTCGCT15550.040.3007932
GTCTTGC27400.037.8857231
CGCTCTG15650.037.723265
CTCGCTC17300.036.224123
TTGCTCT36750.029.3862154
TCGTCCG1050.00797785227.4271359
GTCTTAC12100.027.40541
GTCTCAC31650.026.4209211
GGGTTTC46100.025.4888551
TCTGTCA35450.025.386548
CTTGCTC37950.025.0545653
CTGTTGC33150.024.54179
TCTTGCT41400.024.3586042
GTGTTAG22000.024.247821
GCGTTAC1500.001545431324.0293711
GTTTCAC42650.023.9824753
Produced by FastQC (version 0.11.5)