FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3334350
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13800.040.1779331
TCGCTCT16250.038.5428774
TCTGTCG17400.036.439858
GTCTTGC27750.035.5495031
CTCGCTC16900.033.226623
GTCTTAC9900.032.730551
CGCTCTG17600.031.9051065
TCTCGCT18750.029.184732
GTATTAG9950.028.223931
GTACGGA2054.2924694E-728.100371
TTGCTCT35900.025.8686374
CTGTCGC25450.025.763029
GTCTCAC34550.024.8013271
GTGTTAC11150.024.5405731
GTATTGC9200.024.2632981
AATGGCG17650.024.0649815
ATGGCGT15000.023.9970026
CTTGCTC36450.022.9107193
ACCGTGT9850.022.6761138
GTGTTAG20700.022.6109581
Produced by FastQC (version 0.11.5)