FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2980785
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11050.046.257461
GTCTTGC24700.037.890651
TCGCTCT13950.035.0924034
CGCTCTG13900.033.146955
CTCGCTC14700.032.812243
TCTGTCG13500.032.566988
TCTCGCT14800.031.131782
TTGCTCT31900.028.8866834
GTGTTAG19750.028.0678631
GTATTAC6750.027.7303431
CTTGCTC31400.026.1368713
GTACGGA2551.0168151E-725.4090271
GTCTTAC9750.025.1049861
GTCTCGA26100.025.100326
TTCGCAC1751.3180982E-424.6826423
GTCTCAC31700.023.8460121
TCTGTCA32950.023.8425378
GTAGCAC9700.023.7496193
TCTTGCT37850.023.5853042
CTGTCGC20800.023.5628059
Produced by FastQC (version 0.11.5)