FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4264306
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC17600.038.4556731
GTCTTGC35400.036.407851
TCGCTCT20350.031.8406984
TCTGTCG23650.030.4652358
TTGCTCT46700.028.366484
GTGTAAT14950.027.933931
TCTCGCT23600.027.4577882
CGCTCTG23650.026.484555
CTCGCTC24050.025.74473
GTCTCAC45000.024.8007261
GTCTTAC14550.024.2481331
CTTGCTC46850.023.5118243
CTGTCGC32050.022.7048599
GAGTGCA99700.022.3857176
GGAGTGC92750.022.1225625
CTCGATC16650.022.0694548
GGTATAC7850.022.0133841
GTCTCGA34100.021.9575276
GGGTTTC56950.021.8724581
TCTTGCT54950.021.7508092
Produced by FastQC (version 0.11.5)