FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3488458
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14100.043.4025548
GTCTCGC16100.037.6138841
GTCTTGC30700.036.6337171
CTGTCGC17650.035.4886939
TCGCTCT15300.034.8221634
TCTCGCT17400.032.7071232
GTGTTAG20650.031.0716781
CTCGCTC17750.030.8446433
CGCTCTG16300.028.7105335
TTGCTCT40100.028.5475084
CTCGATC12250.028.211148
GTACTAC5950.027.8679891
TACCCCG3900.027.6912215
GTCTTAC12800.027.0349791
CTTGCTC37600.026.8228383
GTATAAC6000.026.4342021
GTATTAG11800.026.2712781
TCGATCT13200.025.0899249
GTCTCGA31050.025.0424966
GTTAGCC16400.025.0377663
Produced by FastQC (version 0.11.5)