FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3498382
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12700.039.6830338
GTCTCGC15050.039.2744061
TCGCTCT15650.037.7233354
TCTCGCT17200.033.5039182
CTGTCGC14850.033.4528549
GTCTTGC27550.032.1822051
CGCTCTG17900.030.97055
CTCGCTC18050.030.7289433
GTATTAC7400.028.2487051
GTCTTAC12300.027.5438731
GTGCTAC11050.023.4840241
GTAACAC12950.023.362193
TTGCTCT37050.023.1243384
GGTATAC6550.023.1105481
GTGTAAC9700.023.0368041
GTCTCAC37050.022.9575791
GTGTATA20500.022.8555551
GTTTCAC44750.022.3746283
GTACTAC6800.022.2608971
TAACACT15250.021.7169344
Produced by FastQC (version 0.11.5)