FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4010604
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC15000.035.9916081
TCGCTCT16750.030.9640484
TCTGTCG20050.030.189188
GTCTTGC31850.026.6688211
CTCGCTC20200.026.3888473
TCTCGCT20200.025.322822
GTATTAG11350.024.1001051
CTCGATC14700.023.5293718
GTACTAT8400.023.137461
CGCTCTG18750.023.0487145
GTCTCGA34200.022.9560476
GTGTTAG24000.022.7946831
GGTATAC9500.022.7315431
TACACTG22100.022.1622245
GGAGTGC79450.022.1203845
GGGTTTC50850.022.0833451
GTTTCAC47300.021.930143
GAGTGCA88850.021.7257146
GTGTTAC13950.021.6723651
GTATTAC8650.021.6365721
Produced by FastQC (version 0.11.5)