FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7723225
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC63050.034.6025351
GTCTCGC31300.033.3560031
TCGCTCT34100.031.4606864
TCTGTCG37150.029.844518
CTCGCTC36650.027.110743
GTATTAC18850.025.2105851
CGCTCTG37700.024.8278035
GTATTAG25600.024.4697551
TTGCTCT89650.023.933284
GTCTCAC83700.023.5708621
TCTCGCT42550.023.3530652
GTATTAA22800.023.053581
GTGTAAC22450.022.7715381
CTTGCTC81600.022.5001963
GTGTAAG33900.022.3018021
GGAGTGC154550.022.1289545
GAGTGCA170050.021.8460456
GGGTTTC111800.021.7683371
GTGTTAG45300.021.4578361
TCTGTCA81700.021.3253368
Produced by FastQC (version 0.11.5)