FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2943996
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10600.039.3921931
GTCTTGC25250.035.9250341
TCGCTCT12650.033.5952844
TCTGTCG14450.031.4128768
GTATTAG10050.027.9374371
CGCTCTG14000.026.7510265
TCTCGCT15100.026.7201392
CTCGCTC14900.026.5884673
GTGTTAG18950.026.2136731
GGCTAGA15950.025.7277741
GTCCTAC8250.025.3064981
GTGTTAC10550.025.2485981
TTGCTCT32600.024.9676534
GTCTCGA24200.024.4041486
CTTGCTC32200.024.159323
GTCTTAC10600.023.771151
GTATTAC6700.023.639371
TCTGTCA31050.022.9725178
CTAGAGT13200.022.918823
GTCTCAC29750.022.747251
Produced by FastQC (version 0.11.5)