FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3371545
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13050.039.719731
TCTGTCG15950.034.782638
GTCTTGC28100.034.330711
TACGTCG900.003750850232.006425
TCGCTCT15550.030.568794
CTCGCTC15750.030.1806163
TCTCGCT16900.028.9856912
GTCTCAC34100.028.9234731
CGCTCTG16900.025.5672595
TTGCTCT37550.025.5097664
GTGTTAC13150.025.1835171
CCGTGTT10450.024.809549
GTGTTAG22250.024.266971
GTTTCAC43150.024.2020383
CTTGCTC36600.024.0073093
GTACCGC2401.7009115E-623.997341
ACCGTGT9800.023.5264438
TTCACCG10150.023.4135655
GGGTTTC47100.023.233091
GTCTTAC10850.023.2232281
Produced by FastQC (version 0.11.5)