FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3997612
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14800.041.3426328
GTCTCGC15500.034.872361
CTGTCGC19800.033.083939
TCGCTCT16400.031.6169284
GTCTTGC34250.031.1424641
TCTCGCT16950.029.7412622
CTCGCTC18150.026.1877573
CGCTCTG18300.025.9731085
GTACTAA8500.025.4363121
GTCTTAC15100.025.2959351
TTGCTCT46200.025.2524854
GTGTAAC10950.024.3522681
CTTGCTC44700.024.1665573
TCTGTCA40400.023.5197938
GTATTAC9350.023.1239191
GGAGTGC83700.023.0589965
GTCTCAC39350.022.8937591
CTGTTGC37100.022.701419
GTATTAA12500.022.48571
GAGTGCA89900.022.179976
Produced by FastQC (version 0.11.5)