FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2847808
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9150.050.3494958
TCGCTCT11800.037.227124
GTCTTGC23200.035.7275351
CTGTCGC13000.035.4382979
GTCTCGC11050.034.570591
CTCGCTC13250.032.6097153
TCTCGCT14200.031.442352
GTGTAAC9800.027.9479981
TTACGAC1358.410978E-426.6715243
CGCTCTG14550.025.2417135
TTGCTCT31950.025.0186444
GTATTAG11000.024.8991281
GTGTTAA11650.024.7472691
GTGTTAG16700.024.1693361
GGGTTTC39050.023.2564131
GGCTAGA17000.022.8948631
TCGATCT9850.022.6548779
TCACTCT32650.022.4972084
GTACTAT6100.022.4500311
TAGTACT6750.022.4040834
Produced by FastQC (version 0.11.5)