FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2847808
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11900.037.5506138
GTCTTGC21550.037.41511
GTCTCGC11500.033.17831
TCGCTCT12300.032.801014
CTCGCTC12450.028.9337653
CGCTCTG13000.028.8149935
GTGTTAG16250.028.796261
TCTCGCT13450.027.3278182
GTTAGCC13600.027.0169053
GTCTTAC9850.026.3113041
GTATTAA7950.025.3551981
CTTGCTC29900.024.8182053
GTACGGA1751.3181256E-424.682511
GTCTCAC28900.024.4120561
GGGTTTC37800.024.3777891
TTGCTCT32250.023.9033284
TTACACT12150.023.125584
CTGTCGC21250.023.0463229
GTACTAT6900.022.9535431
TAGCCCG2202.3438142E-522.9210175
Produced by FastQC (version 0.11.5)