FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3679196
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13500.047.4651578
CTGTCGC17700.034.5751729
GTCTTGC28700.033.131041
GTCTCGC13850.032.2466741
TCGCTCT15900.029.8858414
GTATTAC8800.027.013091
TCTCGCT16550.026.9844192
GTCTTAC12700.026.6585881
CTCGCTC16650.026.3897323
CGCTCTG17400.025.2405765
GTGTAAC10950.024.3405631
TTGCTCT41450.023.7965894
TTACACT18200.023.7355084
GTGTAGC13800.022.9676511
GTATTAA12650.022.7778361
GTGTTAG26100.022.6316591
TAATACT11600.022.3441184
CTTGCTC38400.022.3221053
GTATTAG13950.022.204311
GTCTCAC36100.021.7501541
Produced by FastQC (version 0.11.5)