FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3046530
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12100.047.0066578
GTCTCGC12550.042.468761
CTGTCGC15600.037.844869
TCGCTCT13300.036.8113754
GTCTTGC25350.032.3898161
GGTATAC5800.031.045141
TCTCGCT15950.030.7049542
GTGTTAC10750.030.1498851
CTCGCTC15600.029.5471523
CGCTCTG15650.029.4435655
GTATAAG8900.025.896531
GTATTAA8350.025.8771481
CTCGATC10200.025.4108988
TTGCTCT33750.025.1728864
GTATTAT9550.024.8881241
GTCTCAC31050.024.5881521
TCGATCT10700.024.223479
CTTGCTC30350.023.9675083
GGAGTGC66550.023.368485
GTGTAGA16800.023.1508051
Produced by FastQC (version 0.11.5)