FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4991558
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG18150.039.2715538
GTCTCGC21200.037.3933331
TCGCTCT23050.035.2961274
TCTCGCT23800.033.300672
GTCTTGC41500.032.8209421
CTGTCGC24150.032.1977969
CGCTCTG25950.032.1840135
CTCGCTC25100.030.4277173
GTGTTAC19650.030.0738261
GTATAGG14400.024.522851
GTCTCAC49600.024.2645451
TTGCTCT54150.024.0657634
GGGTTTC72150.023.8725571
GTTTCAC66700.023.3327083
GGTATAC9400.023.0001541
GTACTAT12050.022.726571
GTATTAC12400.022.6662831
TAATACT15950.022.5698594
GTCTTAC16400.022.4111271
GTGTAAA30400.022.046861
Produced by FastQC (version 0.11.5)