FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3950094
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13750.042.4134378
GTCTCGC15450.037.294741
CTGTCGC19350.033.8595779
GTCTTGC32950.033.6628881
TCGCTCT15450.032.6205064
TCTCGCT18000.028.4075262
CGCTCTG17450.026.8187835
TTGCTCT41550.025.8188194
GTGTTAG25600.025.6028021
GTCTTAC14100.025.5409451
CTCGCTC18650.025.1004813
GTGTTAC14300.024.6800541
CGTTTCG2351.4151701E-624.5099989
GTATTAA12150.024.3048881
GTCTCAC41000.024.2427181
GGGTTTC56500.023.9659941
TTACACT16600.023.8547974
GTTAGCC19000.023.5009633
TACACCG3702.8376235E-1023.3507395
GGAGTGC81950.023.1940215
Produced by FastQC (version 0.11.5)