FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3950094
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17950.040.512518
GTCTCGC15150.040.3970381
GTCTTGC30850.036.6427151
TCGCTCT17800.035.1918754
GTATTAA10450.031.0055471
TCTCGCT20400.029.2963222
CGCTCTG20250.028.4451455
CTCGCTC21250.028.1230453
GTATTAG11850.026.7348351
CTGTCGC27000.026.1323229
GTCTCAC40400.024.2382181
GTGTAAC10250.023.8835141
TTGCTCT42250.023.8585764
GTGTTAC14550.023.7531641
ACCGTGT12150.023.7036888
TCTTGCT46400.023.5879822
GGAGTGC78900.023.3617925
TAATACT11450.023.2669474
GAGTGCA87550.022.6975826
TTACACT16850.022.6474424
Produced by FastQC (version 0.11.5)