FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4527865
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15600.036.921718
GTCTCGC17800.032.392331
CTGTCGC19750.030.621659
GTCTTGC37700.028.6762391
GTACTAC7550.028.6382561
TCGCTCT18350.027.857284
GTATAAC9450.027.456361
TCTCGCT21100.026.2962682
CGCTCTG21550.024.3888875
GTGTTAG30100.023.7050551
GTGTTAC16150.023.6524221
GTATTAG15900.022.6644481
CTCGCTC22050.022.5489523
GTGTAAT19650.022.373791
GTCTCAC44400.022.2387241
GGTATAC10750.022.1247161
GTATTAA14700.022.0631451
GTGTAAG22800.021.8115481
GGGTTAT12300.021.680481
GTTAGCC23050.021.5706883
Produced by FastQC (version 0.11.5)