FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3131425
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11350.036.1569448
GTCTTGC26050.033.7505721
GTCTCGC12200.033.079371
TCGCTCT13000.030.4601524
CTGTCGC14500.030.2882799
GGTATAC6350.027.2374551
TAACCCG2955.2386895E-1026.8462355
GTGTAAA19100.025.6569231
GGCTAGA18650.025.5031661
TAAACCG1701.08200096E-425.4106085
TCTCGCT15500.024.6355442
CGCTCTG14950.024.5607575
GTGTTAG20600.024.1385351
GTATAGC7250.023.8562561
TTGCTCT39400.023.38984
CTCGCTC16150.023.1979053
GTCTTAC12200.023.0374181
GTCTAGC11950.022.9163111
CTTGCTC36900.022.844283
TTACACG3254.3028194E-822.1528384
Produced by FastQC (version 0.11.5)