FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3131425
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12500.040.8988151
TCGCTCT13200.036.001334
TCTGTCG14250.032.8480388
CTCGCTC14450.031.3921643
GTCTTGC27450.031.2152631
GTATTAC7600.030.3178771
TCTCGCT16150.028.9793972
CGCTCTG14500.028.7992235
GGTATAC6800.024.3546181
GGAGTGC62550.023.5964855
GAGTGCA67800.023.5745856
GTGTTAC12250.023.5118221
GTATTAG11250.023.0415881
TTGCTCT36900.023.025244
GTCTTAC10700.022.8800811
CTGTTGC29050.022.5582929
GGCTAGA17650.022.4378071
GTCTCAC31500.022.4015431
CTGGAGT77300.021.7032593
CTGTCGC23900.021.6942969
Produced by FastQC (version 0.11.5)