FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4167913
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14300.040.2788668
GTCTCGC16200.036.919111
TCGCTCT17050.032.937724
CTGTCGC19650.030.7779759
CTCGCTC18000.028.8170223
GTATTAC8950.028.1794911
GTCTTGC34100.027.4711691
TCTCGCT19600.026.8321742
TAATACT13300.023.8190444
GTCTTAC16050.022.8972471
CGCTCTG21000.022.6280945
GTACTAC7700.022.4599461
GTGTTAG27950.022.429811
ACCGTGT12400.021.4834188
GGTATAC9150.021.2633081
GTATAAT14100.020.9533231
GTACTAA10100.020.6902051
GTATAGA14800.020.4491731
GTATTAG15100.020.0428961
GTGCTAA15200.019.9110371
Produced by FastQC (version 0.11.5)