FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4167913
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT16350.030.825474
GTCTCGC16000.030.600151
GTCTTGC31100.028.2445131
TCTGTCG18250.024.856118
CTCGCTC18550.024.4526563
CGCTCTG18750.024.1909565
GGGTACG3952.5465852E-1123.696321
GTCTCAC40150.023.492021
GTACTAG8300.023.4218031
GTGTTAG26800.022.8359341
GTCTTAC14600.022.6850411
GAGTGCA78700.021.6814046
TCTCGCT20700.021.5650652
GTGTTAT16750.021.4926431
GCGTTAC2704.725278E-621.3334391
GTCCTAA12950.021.1275181
GTATTAG12650.021.0593931
GTATTAC8550.021.0527341
GTATAAC8600.020.9303361
GTATTAA12400.020.9033281
Produced by FastQC (version 0.11.5)