FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4058610
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15750.043.4273878
GTCTCGC17750.036.5438651
GTCTTGC33900.035.29221
TCGCTCT19200.033.749084
CTGTCGC20050.031.6001369
TCTCGCT21550.029.0879962
CTCGCTC21650.028.9536423
CGCTCTG21200.028.867145
TTGCTCT47050.026.6262664
CTTGCTC42600.025.201043
GGGTAGT13350.024.2941421
GTGTAAA24800.023.8304631
GTGTAAC12000.023.4236031
TTCACCG11150.023.2460045
CTGTTGC38500.022.8149649
GTTTCAC53900.022.4575483
GTATTAG15450.022.3914951
TACACTG23550.022.3178655
TCTGTCA46600.022.2483228
ACCGTGT14000.022.1136848
Produced by FastQC (version 0.11.5)