FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4058610
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC15950.035.662441
GTCTTGC35600.031.7536981
TCTGTCG20300.031.5689438
TCGCTCT17850.027.4293384
GGGTTTC57400.026.7185171
CGTTTCG2456.8908776E-826.4510259
CTTCGCG1701.08117645E-425.4137279
CGCTCTG19300.025.3673345
TTGCTCT46150.024.4947284
GTGTAAA25600.024.1881771
GTATTAG13550.023.9121071
TCTCGCT22700.023.788922
GTCTCAC41800.023.7711
CTCGCTC20350.023.7058243
GTTTCAC53350.023.618283
GTCTTAC13650.023.209441
GTGTTAC15550.023.1517731
ACCGTGT12150.023.1128988
TACACTG24450.022.085425
CTGTCGC29500.021.9677989
Produced by FastQC (version 0.11.5)