FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3991621
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10250.023.9003588
GTCTCGC10750.023.7722231
TCGCTCT11700.021.8414544
CTGTCGC12450.020.8014039
TGTCGCC12700.020.66749410
TCTCGTA8800.020.28385270
TCTCGCT12700.019.2947372
TCGCCCA13300.019.20885712
GTCGCCC12400.019.1918811
GTATTAA8950.019.1658671
GTCTTAC10100.018.7166351
CTCGCTC12350.018.4242973
GTCGCGA1150.001009201418.25928311
GACTCGC5000.018.19978570
GTATAAT7300.017.7432961
GTATTAT8100.017.2874281
GTATAGA6900.017.2498471
GTGTAAA15850.017.227441
GTGTACG2259.3948984E-817.1145531
CGCTCTG12900.017.0960335
Produced by FastQC (version 0.11.5)