FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3769185
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9250.032.5385638
TGTCGCC11700.024.52842310
CTGTCGC12350.024.3709899
TCGCTCT11250.023.3368684
GTCTTGC23050.022.020981
GTATTAG8550.021.6994821
GTCGCCC11100.021.1248411
GTCTCGC11450.021.0951771
GTACTAA6000.021.003461
CGCTCTG12300.020.7755055
GGTATAC5200.020.1956351
TCGCCCA12250.019.71308712
TATACCG1107.4738305E-419.09385
GTATAGA6900.018.2638781
CTCGCTC13050.018.240313
GTCTTAC10050.017.764121
TCTCGCT12850.017.4347782
GTATTAC6650.017.3712831
TTGCTCT29550.017.2953434
GTATTAT7700.017.2755721
Produced by FastQC (version 0.11.5)