FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4239507
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16700.049.147098
GTCTCGC16900.039.7311861
TCGCTCT17650.037.119844
GTCTTGC36000.034.294851
CTGTCGC23950.033.668759
TCTCGCT19900.031.1355882
CGCTCTG20650.030.332535
GTGTAAC12400.029.695031
GCGTAAC2452.064553E-929.469261
CTCGCTC21250.028.479483
TTGCTCT48600.027.554094
TTACACT20150.025.368384
TACACTG26400.025.3622885
CTTGCTC48100.025.1637823
GTCTCGA34000.024.3516466
GTGTTAG27900.024.3253421
GTGTTAC16700.024.2106721
TCGATCT14300.024.1668329
TAACACT19750.024.059484
TACCCGT3052.1951564E-823.6053036
Produced by FastQC (version 0.11.5)