FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4239507
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT18750.043.7741974
TCTGTCG19800.043.634568
GTCTCGC17600.042.6988031
GTCTTGC34150.038.29861
CTCGCTC21150.037.785233
CGCTCTG21650.035.582835
TCTCGCT22300.033.592542
CTGTCGC29750.028.7988139
CTTGCTC46300.025.9683843
TTGCTCT50000.025.6309434
GGGTTTC58350.025.5106411
TCTTGCT52100.025.0239122
GTGTTAG26350.024.4064391
GTTTCAC57200.024.2924183
GTCTTAC16800.024.0865061
GTACTAT9350.023.95771
GTATTAC10000.023.845641
GTCTCAC42750.023.6639331
TAATACT13950.022.7087384
CCGTGTT13350.022.650759
Produced by FastQC (version 0.11.5)