FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3825833
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10050.026.4664698
GTCTCGC12050.023.821961
TGTCGCC10800.023.00822310
CTGTCGC11550.021.81729
TCGCTCT12000.021.0039964
GTCTTGC22000.021.0039961
GTACCGT1408.245932E-620.0038071
GTATTAG8550.019.6528631
GTCGCCC11400.019.6482411
TACACTG18150.019.0945425
GTATTAA7750.018.5196511
ATCTCGT13250.018.48997770
GTGTTAG16050.018.3212431
TCGCCCA13400.018.28275912
CTCGCTC13600.018.0181353
TCTCGCT14400.017.9895342
GGATAAT10650.017.7498551
TATACTG7750.017.6162535
TTACACT13650.017.1827564
CGCTCTG13100.017.1024915
Produced by FastQC (version 0.11.5)