FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3974106
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7600.027.1692378
TGTCGCC10800.021.06340610
CTGTCGC10950.020.7748669
GTACTCG1408.2437655E-620.004451
GTCTCGC10200.019.9063911
GTCTTGC24150.019.4246121
ATCTCGT14450.019.1339170
GTACCGT1301.056765E-418.8501116
GTCGCCC9550.018.32338111
TCGCTCT10900.017.9854284
GTACTAG5650.017.96861
TATACTG8850.017.8003465
GTATAGA7900.017.7254621
GGGTAAT9400.017.5038971
GTGTAAA15800.017.2823261
CGCTCTG11200.016.8785445
GTATTAC6850.016.8650671
GTGTATA13700.016.8650671
GTATTAA8650.016.5932881
GTATAGT7150.016.1574421
Produced by FastQC (version 0.11.5)