FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5716134
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG19750.049.9414188
GTCTCGC23050.040.7218671
TCGCTCT24100.038.8372884
CTGTCGC29100.036.369359
GTCTTGC48600.035.9529341
CGCTCTG25850.033.700185
TCTCGCT28200.031.9432262
CTCGCTC29000.031.310533
TTGCTCT64800.026.7771684
CTTGCTC60050.025.561393
GTCTCAC55300.024.2852751
GGGTTTC80050.024.1728971
GTGTTAG36150.023.96781
TCGATCT20750.023.9409969
ACCGTGT16050.023.774338
GTCTTAC21000.023.723841
GTTTCAC72350.023.5066833
CTCGATC21700.023.2244668
TCTCGAT21350.022.930767
GTCTCGA45000.022.3987066
Produced by FastQC (version 0.11.5)