FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5716134
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC21050.043.24211
TCGCTCT25350.038.624714
TCTGTCG26750.035.7958038
GTCTTGC43500.034.7092631
CTCGCTC26800.034.3858223
CGCTCTG27950.031.9406785
TCTCGCT28800.031.2641052
TTGCTCT61400.026.1481364
GTGTTAG32850.025.7299461
GTTTCAC68350.025.701333
GGGTTTC74300.024.8908371
GTGTTAC21900.024.410461
CTGTCGC41450.023.9694929
TAATACT17200.023.8589134
CTTGCTC60100.023.8387173
GTCTTAC20300.023.487471
TACACTG31700.023.3928015
GTTAGCC26050.023.2153823
GGAGTGC112950.023.201685
TCTGTCA60650.023.147718
Produced by FastQC (version 0.11.5)