FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2561879
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8800.047.785628
GTCTCGC9600.037.8636171
GTATACG1051.8250148E-434.6181641
GTCTTGC19750.032.3920821
GTATTAG8600.031.2771111
TCGCTCT10600.030.7955174
CTGTCGC14250.030.5272949
CGTCGTT950.004727919630.5272927
GTCTCAC23250.030.0172981
GGTATAC3400.029.934531
TCTCGCT11750.029.637062
GTGTTAT8650.029.4154361
GTACCGA1000.00599571829.0792581
CGCTCTG10750.028.9986675
CTCGCTC11800.028.277973
TAATCCG1306.4874825E-427.8833315
ACGTCGT1100.00967283826.3644816
TTGCTCT27450.025.8979024
GTGTTAG14750.025.6291771
GTATAGA5700.025.5081211
Produced by FastQC (version 0.11.5)