FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2561879
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8950.040.777051
TCTGTCG10350.036.4255268
GTCTTGC18100.033.874241
TCGCTCT10100.033.7347074
GTATAAG6300.031.2818241
TAGCGCG950.00473065930.5236784
GTGTTAG12700.029.3113161
CTCGCTC11350.028.1043953
CGCTCTG10900.027.9360925
ACTCGTT1306.4853113E-427.8849287
GTACTAG4200.027.8060671
CTGTCGC13100.027.6720649
TCTCGCT12150.027.4477962
GTTAGCC11500.027.1074123
TTGCTCT25150.026.2303374
GTATAAC3657.2759576E-1225.9967651
GTATAGG4850.025.5844461
GTATTAA6650.025.244981
GTACTAT4950.025.067591
GTGTAAA14050.024.9364011
Produced by FastQC (version 0.11.5)