FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2582150
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7850.039.7128078
TCGCTCT9600.037.771584
TCCGTCG800.00203735436.249488
TAGACCG1301.6559537E-533.461065
GTCTCGC9650.033.1315841
GTCTTGC17650.032.1119541
GTCTTAC6150.031.9010871
CGCTCTG9650.031.5539535
CGCGCAA1204.0601476E-430.207901145
GTATTGG5950.029.3096851
GTGTATA6450.029.2907491
GTGTTAG11500.028.433581
GTATTAG6400.028.3842141
GTATTAA5900.028.3264851
TTATACT5300.027.3665774
CTCGCTC11550.026.99883
CTGTCGC11400.026.7101449
TCCTACG1658.4011794E-526.3739852
TAGTTCG1100.00966043526.3714314
GTATTAT5850.026.0843661
Produced by FastQC (version 0.11.5)