FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2582150
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9900.041.7416768
GTCTCGC9350.033.575061
GTCTTAC7800.032.7592121
TCGCTCT10000.031.8981934
CTGTCGC13150.030.8739729
CGTTTCG1204.0602338E-430.2077929
GTCTTGC16200.030.193941
GTGTTAG12350.030.1483231
CTCGCTC11250.029.643343
GTATATA7000.029.2024961
CGCTTAG1254.950281E-429.2024961
ATACCGG1255.156612E-428.9994816
TTAAACG2003.2593198E-728.9983584
TCTCGAT8850.028.6718047
CTCGATC8950.028.3514488
GTTCTAC4700.027.9598371
GTACGGA1050.007453220427.8119011
GTGTAAT7900.026.7997591
GTCTAGT6550.026.7503781
GTGTTAA6950.026.2612381
Produced by FastQC (version 0.11.5)