FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2522005
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8500.043.4992878
GTCTTGC16750.036.0183331
GTGTTAG10950.035.1821251
GTCTCGC9150.034.9535981
TACCGGT1051.8546928E-434.5232437
TTATACT6300.033.3850444
GGTATAC3350.032.5466841
GTCTTAC6950.032.4218061
TACCCGT1352.1443093E-532.2204175
GTATTAT6100.030.98161
CTGTCGC12350.030.5258169
TCGCTCT10500.030.3919034
GGGTAAA6650.029.5122571
GTATTGG4800.028.7721731
GGATAAT6650.028.419211
CGCTCTG10000.028.2734135
GTTAGCC9500.028.247023
GTGTTAA6700.028.2071271
GTATAAG5700.026.7796421
CTCGCTC10900.026.6150953
Produced by FastQC (version 0.11.5)