FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2440855
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9150.048.3301358
GTCTCGC8600.046.6797331
GTCTTGC15200.041.2970471
TCGCTCT10900.034.584164
GTCTACG1707.3803676E-834.3482971
CTGTCGC13400.031.919539
CTCGCTC11250.030.9325543
GGTTACG950.00473014630.5242969
GTGTTAC6400.029.652241
CGCTCTG11150.029.2581545
GTATTAA4500.029.1960531
TCTCGCT12750.029.000462
CGTTTCG1000.006078111528.9980839
ATACCGT1000.006078111528.9980836
GTATAGA5450.028.1246361
TCGAACT14200.028.079139
GGATAGG6500.028.0731281
TCTACGG1555.50303E-528.064962
GTATAGG3650.027.9962161
CTTACGA1306.4881553E-427.8827729
Produced by FastQC (version 0.11.5)