FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2845423
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8350.049.4895868
GTATCGG752.5132267E-548.4579621
TCGCTCT10450.043.0297434
GTCTCGC10100.042.460691
TCTCGCT11850.036.7265132
GTCTTGC20200.034.9041251
CTGTCGC12100.034.1519059
CTCGCTC11850.033.6618273
CGCTCTG11700.032.840355
GTATTAA5850.032.3053051
GTCGTAC900.00358386632.3053051
GCGTAAT900.00358386632.3053051
GTATAGC5500.030.3963571
GTGTTAG13900.029.8068751
TTGCTCT26350.029.175524
GTCTCAC23050.027.7503281
TAGCACT11400.027.3562764
GTGTATA9300.027.3552991
GGCTAGA14850.026.9210911
CTTGCTC25150.026.5303633
Produced by FastQC (version 0.11.5)