FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2362119
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8050.038.992791
TCGCTCT10200.034.8235134
GTACTCG1707.373092E-834.3520621
CGCTCTG9800.034.0286875
GTCTTGC16600.033.4208341
TCTGTCG9400.032.3931588
ACGCGAA900.003628346632.223816145
GTCTTAC6850.030.904321
TAGCGTA950.00472891330.5258839
GTCTACG1203.9000486E-430.415891
CTCGCTC11450.030.3906523
TCTCGCT11500.029.6293892
GTGTTAG12200.028.720581
CCGTTAT1050.007457134327.8088111
GTACTAT3950.027.720811
GTATAGA6700.026.1485861
GTGTAAG8400.026.070761
GTATAAG4800.025.8535061
TGTACCG1450.001227342624.9985875
CTGTCGC13000.024.5381159
Produced by FastQC (version 0.11.5)