FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2270260
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7500.039.6320538
GTCTTGC19500.036.5006371
TCGCTCT8100.035.807624
GTCTCGC8950.035.705791
CTGTCGC10550.030.2359899
TTATACT6800.029.8572354
GTCCGCG1255.1025813E-429.0515291
AGTACCG1753.7783466E-628.9990625
TCTCGCT11000.027.6900712
CTCGCTC9300.027.2888723
GTCTCAC22750.026.4975471
GTATAGT5550.026.1725481
CGCTCTG11450.025.9598585
GTCTTAC7900.025.7418611
GTGTAGT8300.024.501291
GTATTAG6250.024.4032841
GCCGTAC1500.001479481824.2096061
GTGTAAT8500.023.9247881
TCACTCT26000.023.4264474
GTCTAGT9050.023.2733231
Produced by FastQC (version 0.11.5)