FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2211010
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8150.044.479458
GTCTCGC8550.033.9803121
GTCTTGC17350.032.6534161
TATACGG900.003620774332.2374732
TATCGAT900.003620774332.2374732
TCGCTCT9550.031.1340454
GTATATA6950.029.2621821
GTGTTAG11450.029.1800351
GTATAAA7350.028.6578871
CTGTCGC13350.028.2402869
TCACCGT5200.027.8851936
GGACGAT2603.7234713E-927.8851936
GTTAGCC9750.027.5196153
GTATAAT5050.027.3272361
ACCGTGT5100.027.0103658
TTGCTCT22300.026.6663784
TCTCGCT10450.026.3761142
TTCACCG4950.026.3629915
CTCGCTC10700.025.754023
GTACAGA8350.024.3559461
Produced by FastQC (version 0.11.5)