FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2707556
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAC901.3069784E-648.426391
TCTGTCG9150.046.7469568
GTCTCGC10300.045.840521
TCGCTCT11600.043.140184
GTCTTGC19300.038.3898351
CGCTCTG12300.036.5427255
TCTCGCT12650.034.9760132
CTGTCGC13100.034.8651589
CTCGCTC12550.033.5177733
GTGTTAG13550.031.0929621
GTCTTAC7550.030.787641
GCGCGAA1453.4934317E-529.998922145
TTGCTCT23150.028.1956674
CGCATAA1306.4871943E-427.883614145
GTCTAGG8800.027.2398451
GGAGTGC53200.026.4365315
TATACTG6350.026.2584135
TTACACT8600.026.1429214
GAATTAT9550.025.8612161
GGGTAAT5900.025.8547691
Produced by FastQC (version 0.11.5)