FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2707556
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAC7600.040.332251
GTCTCGC8800.037.3204231
GTCTTGC17750.036.5938721
TACGAGT1209.576288E-636.2464644
TCTGTCG9650.036.0639888
GTACTAA4100.032.040951
TAATACT5150.030.9678524
TCGCTCT11150.030.5575584
GTTCTAC4600.030.1448061
GTATTAA5550.028.9298651
GTCTCAC20950.028.9141731
TTGCTCT24250.028.6982334
GTATAGG4600.028.5582391
CTGTCGC13500.027.9273269
CTTGCTC22850.027.2849983
GAGTGCA56050.027.0352556
GGCTAGA10850.026.9058671
CTACGCA2705.5697456E-926.8531999
CGCGAAA1357.9867474E-526.852207145
GTCCAGT10900.026.7824441
Produced by FastQC (version 0.11.5)