FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2444542
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC15750.038.0053831
TCTGTCG9250.036.0537578
GTGTTAG12950.035.512611
GTCTCGC9000.034.8768921
TCGCTCT9650.031.551564
TAATACT5350.029.8103984
GTATTAG6650.028.5406281
GTATTGG5700.028.1747231
TTGCTCT23200.027.497524
GTATAAG4300.027.16211
CTCGCTC10750.026.9754163
TATCCCG1907.162569E-626.7097595
GCGCGAA1100.00967369226.363956145
CTGTCGC12350.025.8297449
GTTAGCC11250.025.7765083
GGGTATA6550.025.6329381
GTGCGGA3159.567884E-1025.4914151
TTATACT5500.025.0431984
CTGTTGC19500.024.910059
GTATAAA8500.024.905251
Produced by FastQC (version 0.11.5)