FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3672643
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATC21250.028.16167670
GTTCGTA700.001228032224.9966311
TGTAGAT35800.020.91953870
TCTGTCG10400.020.192618
GTCTCGC9200.020.1726841
GTATTAG5600.020.0095631
TCGCTCT11150.018.520714
CGCCGTA1903.0144656E-718.41932362
CTGTCGC12350.017.5711149
TGTCGCC12350.017.57111410
GTATAAG6800.017.5083681
GTCTTGC20150.017.204251
CGTATCA2056.686787E-717.07156865
GTTAACG1452.4322614E-416.904631
GTACGGG1452.4322614E-416.904631
GCGTAGA1250.001757957616.8080331
GTGTAGA45050.016.46881769
TCGCCCA12400.015.80453612
TCTCGCT11500.015.5211272
GTCGCCC11800.015.42165111
Produced by FastQC (version 0.11.5)