FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2335711
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7100.039.8229878
TCGCTCT7600.036.2475784
GTCTTAC7350.033.765031
GTCTCGC7600.033.6147581
GTACTAT4500.032.440911
GTATAGA6150.030.8584271
GTCTAAT5000.030.656661
GTACGGC950.004729626730.524933
TTACACT8600.030.3468074
TATAGCG1204.0587352E-430.2095472
GTCTTGC16050.030.015421
GTGTTAC6150.029.6715641
TACACTG10050.028.1336525
GTATAGG4250.027.4793571
GTCTCAC20600.026.9291041
GTATTAT4950.026.5425621
TCGAACT10750.026.3016939
TTACTCT11150.026.007234
ATCGATT1400.001000000425.8922357
GTATAAG5100.025.7618981
Produced by FastQC (version 0.11.5)