FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2372261
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7350.051.2909438
GTCTTGC17000.038.8912431
CGTCGAG1157.1483537E-637.8249247
CTGTCGC10900.034.5860949
TTGCTCT24650.029.1314894
GTATAGA5250.029.061591
TCGCTCT9200.027.5946354
CTTGCTC23450.026.9104423
GTGTTAG13650.026.0809141
GTCTCGC8500.025.6425781
GTATATA7100.025.5823861
GGAGTGC42900.025.3483415
GTATTAT4900.025.2064821
GTCCTAC5500.025.0986481
GTACAGA10400.024.4508571
GGGTTTC33400.024.363011
GAGTGCA46350.024.2441336
GTTCTAC6050.024.0178431
GTCTTAC6400.023.8395841
GTATTGG5250.023.5260491
Produced by FastQC (version 0.11.5)